Ayhan, D. Gonullu and Aslan, S. Saglik (2021) Simultaneous Determination of Tinidazole and Lidocaine in Pharmaceutical Preparations by HPLC with Photodiode Array Detection. Journal of Pharmaceutical Research International, 33 (47B). pp. 10-16. ISSN 2456-9119
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Abstract
A selective, simple and new reversed phase high performance liquid chromatographic method (HPLC) was developed and validated for simultaneous determination of tinidazole (TIN) and lidocaine (LID) in the ovule dosage form in this study. Developed method was performed with gradient elution by getting C18 (250x4.6mm,5µm) reversed phase HPLC column, mobile phase containing 10mM potassium phosphate buffer (pH3.0), acetonitrile at 1.0 mL/min. Temperatures for column and autosampler were adjusted at 25Cº. The chromatographic separation was carried out at 220 nm wavelength. Retention times were found as 6.5 min for LID and 8.2 min. for TIN. The purity of each substance was evaluated getting a photodiode array detector. The linearity ranges were 75.0-195.0 µg/mL for TIN and 25.0-65.0 µg/mL for LID. The limit of dedection (LOD) and the limit of quantitation (LOQ) results were 0.05625 µg/mL and 0.225 µg/mL for TIN, 0.01875 µg/mL and 0.075 µg/mL for LID, respectively. The simple, sensitive and reproducible method was applied for simultaneous determination of TIN and LID in pharmaceutical preparations successfully.
Item Type: | Article |
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Subjects: | West Bengal Archive > Medical Science |
Depositing User: | Unnamed user with email support@westbengalarchive.com |
Date Deposited: | 10 Feb 2023 10:29 |
Last Modified: | 29 Jun 2024 12:25 |
URI: | http://article.stmacademicwriting.com/id/eprint/60 |