Zhang, Xiao-zuo and Huo, Hai-qin and Zhu, Yu-qing and Feng, Hao-yang and Jiao, Jiao and Tan, Jian-xin and Wang, Yan and Hu, Ping and Xu, Zheng-feng (2022) Folic Acid Rescues Valproic Acid-Induced Morphogenesis Inhibition in Neural Rosettes Derived From Human Pluripotent Stem Cells. Frontiers in Cellular Neuroscience, 16. ISSN 1662-5102
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Abstract
The ability of human pluripotent stem cells (hPSCs) to specialize in neuroepithelial tissue makes them ideal candidates for use in the disease models of neural tube defects. In this study, we cultured hPSCs in suspension with modified neural induction method, and immunostaining was applied to detect important markers associated with cell fate and morphogenesis to verify the establishment of the neural tube model in vitro. We carried out the drug experiments to further investigate the toxicity of valproic acid (VPA) exposure and the potential protective effect of folic acid (FA). The results demonstrated that neural rosette undergoes cell fate speciation and lumen formation accompanied by a spatiotemporal shift in the expression patterns of cadherin, indicating the model was successfully established. The results showed that VPA caused morphogenesis inhibition of lumen formation by altering cytoskeletal function and cell polarization, which could be rescued by FA supplement.
Item Type: | Article |
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Subjects: | West Bengal Archive > Medical Science |
Depositing User: | Unnamed user with email support@westbengalarchive.com |
Date Deposited: | 03 Apr 2023 07:42 |
Last Modified: | 21 Sep 2024 04:21 |
URI: | http://article.stmacademicwriting.com/id/eprint/411 |