Comparitive Study of Glutathione-S-Transferase Isoenzyme and Vitamin D Levels in Smokers and Non-smokers

Background: In developed countries, cigarette smoking is the leading preventable cause of morbidity and mortality. In the second half of this century, dramatic changes in the prevalence of cigarette smoking in the United States reduced current smoking levels to approximately one quarter of the adult population, reducing gender differences in smoking prevalence and smoking-attributable diseases. Cigarette smoking is a serious risk factor for lung cancer, which is the leading cause of cancer-related deaths in both men and women in the United States and around the world.

Aim: Comparative Study of Glutathione-S-Transferase Isoenzyme mu and Vitamin-D Levels in Smokers and Non-Smokers.

Materials and Methods: A total of 100 people aged 20 to 55 years old who came to Shalinitai Meghe hospital in Nagpur for a health check-up were chosen for the research. Non-smokers make up the control group, while smokers make up the research group. There are 50 patients in each group. ELISA was used to determine vitamin D status. An enzyme-linked immunosorbent assay was used to detect GST-µ in heparinized whole blood.

Results: GST-µ was found to be mostly positive in smokers, and it was also found to be raised in heavy smokers (6.39±3.2) than light smokers (4.56±0.78). GST-µ is positive in light smokers. GST-µ is nearly equal in smokers (5.24±0.95) and heavy smokers relative to others.

Conclusion: Quitting smoking for a longer period of time was related to higher vitamin D levels than current smoking. Furthermore, the GST-µ measure used in our research may be used to show differences in cytogenetic damage between smokers who have a genetically defined detoxification enzyme and those who do not.